The Evaluation of Radio-sensitivity Effect of Hydroxyapatite Nanopartical on MCF-7 and Fibroblast Cell Line

Introduction: Hydroxyapatite nanoparticles inhibit the growth of various cancer cells. The inhibitory effect of these nanoparticles on breast cancer cells of mcf7 has also been reported. However, no studies have been done on the effect of the hydroxyapatite nanoparticles on the radiation sensitivities of the MCF7 cell Line. Our goal in this study is to investigate the effect of cytotoxicity and radiation sensitization of hydroxyapatite nanoparticles on mcf7 and healthy fibroblast cells. In the concentration-dependent process, these nanoparticles greatly inhibit the growth of MCF7 cells compared to healthy fibroblast cells. the radio sensitivity effect of these nanoparticle was also found to be very high in MCF7 cells. Breast cancer is the most common cancer in women. the most common treatment are chemotherapy and radiotherapy after surgery that both of these methods are associated with many side effects. also, many cancer cells show resistance to these two methods. So, researchers are looking for materials that are used to improve cancer with fewer side effects. According to recent advances in the field of nanotechnology, application of nanoparticles in medicine, in particular, the treatment of incurable diseases including cancer treatment, are considered. One of these nanoparticles is hydroxyapatite with the chemical formula, Ca10 (PO4) 6 (OH) 2. Hydroxyapatite is similar to minerals in bones and hard tissues, and has great compatibility with body (Harirchi, Kolahdoozan, et al., 2011). So, it could be used in the different biological and biomedical applications. Materials and Methods: Materials used in this study include: MCF7 Cell Line، Fibroblast Cell، RPMI 1640، DMEM-High glucose، FBS، Pen/Strep، PBS، MTT، DMSO، Giemsa Stain، Etydiombrumid/Acrialorange، Methanol، Trypsin- EDTA، Isopropranol. In this study, the effect of cytotoxicity of hydroxyapatite nanoparticles on mcf7 and fibroblast cells was investigated using the MTT assay at concentrations (0، 12.5، 25،50، 100، 200 and 400µg/ml) of nanoparticles. The radiosensitivity effect of these nanoparticles on the cells was studied with the three methods: MTT assay، clonization and apoptosis assays Results: The study of cytotoxic effects of hydroxyapatite nanoparticles on MCF7 and fibroblast cells by MTT showed that nanoparticles reduce the cell survival in the dose- dependent process and the cytotoxic effects of these nanoparticles are not time-depende. Investigating the effect of hydroxyapatite nanoparticle radiation sensitization on nanoparticles shows that these nanoparticles increase the radiological sensitivity of cancerous and healthy cells, while the amount of this sensitivity in cancer cells is more than healthy cells. Another finding is that hydroxyapatite nanoparticles cause apoptosis in cells. Conclusion: The present study shows the potential of hydroxyapatite nanoparticles as a radiation sensitizing agent in the treatment of breast cancer. Further studies are needed to suggest these nanoparticles as a therapeutic agent.